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cd64 antibody, anti-human, reafinity  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec cd64 antibody, anti-human, reafinity
    Cd64 Antibody, Anti Human, Reafinity, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd64/CD64+Antibody%2C+anti-human%2C+REAfinity/custom%40130-124-325%4042405950
    Average 93 stars, based on 12 article reviews
    cd64 antibody, anti-human, reafinity - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Staining:

    Article Title: Chronic alcohol consumption enhances the differentiation capacity of hematopoietic stem and progenitor cells into osteoclast precursors
    Article Snippet: .. To stain the cells for spectral flowcytometry, the following antibodies were used; CD3 (BUV496, OKT3, BD Biosciences), CD20 (BUV496, 2h7, BD Biosciences), CD14 (BUV805, MSE2, BD Biosciences), CD45RA (BUV395, 5H9, BD Biosciences), CX3CR1 (RB780, 2A9-1, BD Biosciences), NF-κB p65 (pS529) (PE, Kl 0-895.12.50, BD Biosciences), CD184 (CXCR4) (BV480, 12G5, BD Biosciences), HLA-DR (Spark VioletTM 538, L243, Biolegend), CD16 (APC/FireTM 750, 3G8, Biolegend), CD34 (APC/Cyanine7, 561, Biolegend), CD282 (TLR2) (PE/Cyanine7, TL2.1, Biolegend), CD115 (CSF-1R) (PE/DazzleTM 594, 9-4d21ef, Biolegend), SPI1 (PU.1) (Alexa Fluor® 647, 7C6B05, Biolegend), CD64 (VioBlue, 10.1.1, Miltenyi), TNF RI/TNFRSF1A (PE/Cy5.5, H398, Novusbio), CCR2 (APC, 48607, Novusbio), HIF-1 alpha (Alexa Fluor® 488, H1alpha67, Novusbio), CD38 (PerCP or PE/Cyanine7, AT1, Novusbio), CXCR1/IL- 8RA (Alexa Fluor® 700, 42709, Novusbio), CD123 (Brilliant Ultra VioletTM 737, 6H6, eBioscience), CD90 (Thy-1) (Brilliant Ultra VioletTM 563, eBio5E10 (5E10), eBioscience), CD127 (Super BrightTM 780, eBioRDR5, eBioscience), CD284 (TLR4) (Super BrightTM 600, HTA125, eBioscience), TNF alpha (Brilliant VioletTM 650, MAb11, eBioscience), IL1R2 (PE, 34141, Thermofisher), and IRF8 (PerCP-eFluorTM 710, V3GYWCH, eBioscience). .. In order to assess the presence of osteoclast precursors, the bone marrow samples were stained with the following antibodies; HLA-DR (BV605, L243, Biolegend), CD123 (FITC, 6H6, Thermofisher Scientific), CD34 (APC-cy7, 561, Biolegend), CD115 (CSF1R) (PE, 9- 4D2-1E4, Biolegend), CD11b (PE-Cy7, CICRF44, Biolegend), TREM2 (Alexa FluorTM 700, 237920, R&D Systems), RANK (Alexa FluorTM 647, H-7, Santa Cruz), and CD14 (Pacific Blue, MSE2, BD Biosciences).

    Article Title: Non-redundant functions of group 2 innate lymphoid cells.
    Article Snippet: Katja J. Jarick, Patrycja M. Topczewska, Manuel O. Jakob, Hiroshi Yano, Mohammad Arifuzzaman, Xuemei Gao, Sotiria Boulekou, Vladislava Stokic-Trtica, Pierre S. Leclère, Alexandra Preußer, Zoe A. Rompe, Anton Stamm, Amy M. Tsou, Coco Chu, Frederik R. Heinrich, Gabriela M. Guerra, Pawel Durek, Andranik Ivanov, Dieter Beule, Sofia Helfrich, Claudia U. Duerr, Anja A. Kühl, Christina Stehle, Chiara Romagnani, Mir-Farzin Mashreghi, Andreas Diefenbach, David Artis & Christoph S. N. Klose1 ✉

    Article Title: Overcoming the Inflammatory Stage of Non-Healing Wounds: In Vitro Mechanism of Action of Negatively Charged Microspheres (NCMs)
    Article Snippet: .. After Fc receptor blockade, cells were stained with anti CD14, CD64, CD83 and CD206 fluorescent antibodies, washed and analyzed by multicolor flow cytometry (MACSQuant 10, Miltenyi Biotec, Madrid, Spain). .. Macrophages were gated according to their forward- and side-scatter characteristics and data were analyzed by MACSQuantify software (Miltenyi Biotec, Bergisch Gladbach, Germany).

    Article Title: Chronic Alcohol Consumption Enhances the Differentiation Capacity of Hematopoietic Stem and Progenitor Cells into Osteoclast Precursors.
    Article Snippet: E-mail: ilhem.messaoudi@uky. edu.. Chronic alcohol consumption (CAC) is associated with an enhanced risk of bone fracture, reduced bone density, and osteoporosis.. Previous studies, using a rhesus macaque model of voluntary ethanol consumption, have shown that CAC induces functional, transcriptomic, and epigenomic changes in hematopoietic stem and progenitor cells (HSPCs) and their resultant monocytes/macrophages, skewing them toward a hyper-inflammatory response.

    Clinical Proteomics:

    Article Title: Chronic alcohol consumption enhances the differentiation capacity of hematopoietic stem and progenitor cells into osteoclast precursors
    Article Snippet: .. To stain the cells for spectral flowcytometry, the following antibodies were used; CD3 (BUV496, OKT3, BD Biosciences), CD20 (BUV496, 2h7, BD Biosciences), CD14 (BUV805, MSE2, BD Biosciences), CD45RA (BUV395, 5H9, BD Biosciences), CX3CR1 (RB780, 2A9-1, BD Biosciences), NF-κB p65 (pS529) (PE, Kl 0-895.12.50, BD Biosciences), CD184 (CXCR4) (BV480, 12G5, BD Biosciences), HLA-DR (Spark VioletTM 538, L243, Biolegend), CD16 (APC/FireTM 750, 3G8, Biolegend), CD34 (APC/Cyanine7, 561, Biolegend), CD282 (TLR2) (PE/Cyanine7, TL2.1, Biolegend), CD115 (CSF-1R) (PE/DazzleTM 594, 9-4d21ef, Biolegend), SPI1 (PU.1) (Alexa Fluor® 647, 7C6B05, Biolegend), CD64 (VioBlue, 10.1.1, Miltenyi), TNF RI/TNFRSF1A (PE/Cy5.5, H398, Novusbio), CCR2 (APC, 48607, Novusbio), HIF-1 alpha (Alexa Fluor® 488, H1alpha67, Novusbio), CD38 (PerCP or PE/Cyanine7, AT1, Novusbio), CXCR1/IL- 8RA (Alexa Fluor® 700, 42709, Novusbio), CD123 (Brilliant Ultra VioletTM 737, 6H6, eBioscience), CD90 (Thy-1) (Brilliant Ultra VioletTM 563, eBio5E10 (5E10), eBioscience), CD127 (Super BrightTM 780, eBioRDR5, eBioscience), CD284 (TLR4) (Super BrightTM 600, HTA125, eBioscience), TNF alpha (Brilliant VioletTM 650, MAb11, eBioscience), IL1R2 (PE, 34141, Thermofisher), and IRF8 (PerCP-eFluorTM 710, V3GYWCH, eBioscience). .. In order to assess the presence of osteoclast precursors, the bone marrow samples were stained with the following antibodies; HLA-DR (BV605, L243, Biolegend), CD123 (FITC, 6H6, Thermofisher Scientific), CD34 (APC-cy7, 561, Biolegend), CD115 (CSF1R) (PE, 9- 4D2-1E4, Biolegend), CD11b (PE-Cy7, CICRF44, Biolegend), TREM2 (Alexa FluorTM 700, 237920, R&D Systems), RANK (Alexa FluorTM 647, H-7, Santa Cruz), and CD14 (Pacific Blue, MSE2, BD Biosciences).

    Article Title: Chronic Alcohol Consumption Enhances the Differentiation Capacity of Hematopoietic Stem and Progenitor Cells into Osteoclast Precursors.
    Article Snippet: E-mail: ilhem.messaoudi@uky. edu.. Chronic alcohol consumption (CAC) is associated with an enhanced risk of bone fracture, reduced bone density, and osteoporosis.. Previous studies, using a rhesus macaque model of voluntary ethanol consumption, have shown that CAC induces functional, transcriptomic, and epigenomic changes in hematopoietic stem and progenitor cells (HSPCs) and their resultant monocytes/macrophages, skewing them toward a hyper-inflammatory response.

    Incubation:

    Article Title: Formyl-Peptide Receptor Activation Enhances Phagocytosis of Community-Acquired Methicillin-Resistant Staphylococcus aureus.
    Article Snippet: .. Subsequently, the supernatant was discarded, and the neutrophils were incubated with PE-labelled antibodies against CD11b (BD Pharmingen), CD35 (Miltenyi Biotech), CD64 (Miltenyi Biotech) or an IgG isotype control (Miltenyi Biotech) for 30 minutes on ice. .. Next, the neutrophils were fixed with 3.7% formaldehyde, and the fluorescence intensity of the neutrophils was determined using a BD FACSCalibur instrument, and the mean fluorescence intensity (= % PE positive neutrophils × PE fluorescent mean) was calculated.

    Article Title: Probiotic Lactobacilli activate Formyl-Peptide Receptor 2
    Article Snippet: .. Subsequently, the supernatant was discarded, and then neutrophils were incubated with PE-labeled antibodies against CD11b (BD Pharmingen), CD35 (Miltenyi Biotech), CD64 (Miltenyi Biotech), or an IgG isotype control (Miltenyi Biotech) for 30 minutes on ice. .. Next, the neutrophils were fixed with 3.7% formaldehyde, and the fluorescence intensity of the neutrophils was determined using a BD FACSCalibur instrument, and the mean fluorescence intensity was analyzed.

    Control:

    Article Title: Formyl-Peptide Receptor Activation Enhances Phagocytosis of Community-Acquired Methicillin-Resistant Staphylococcus aureus.
    Article Snippet: .. Subsequently, the supernatant was discarded, and the neutrophils were incubated with PE-labelled antibodies against CD11b (BD Pharmingen), CD35 (Miltenyi Biotech), CD64 (Miltenyi Biotech) or an IgG isotype control (Miltenyi Biotech) for 30 minutes on ice. .. Next, the neutrophils were fixed with 3.7% formaldehyde, and the fluorescence intensity of the neutrophils was determined using a BD FACSCalibur instrument, and the mean fluorescence intensity (= % PE positive neutrophils × PE fluorescent mean) was calculated.

    Article Title: Probiotic Lactobacilli activate Formyl-Peptide Receptor 2
    Article Snippet: .. Subsequently, the supernatant was discarded, and then neutrophils were incubated with PE-labeled antibodies against CD11b (BD Pharmingen), CD35 (Miltenyi Biotech), CD64 (Miltenyi Biotech), or an IgG isotype control (Miltenyi Biotech) for 30 minutes on ice. .. Next, the neutrophils were fixed with 3.7% formaldehyde, and the fluorescence intensity of the neutrophils was determined using a BD FACSCalibur instrument, and the mean fluorescence intensity was analyzed.

    Flow Cytometry:

    Article Title: Overcoming the Inflammatory Stage of Non-Healing Wounds: In Vitro Mechanism of Action of Negatively Charged Microspheres (NCMs)
    Article Snippet: .. After Fc receptor blockade, cells were stained with anti CD14, CD64, CD83 and CD206 fluorescent antibodies, washed and analyzed by multicolor flow cytometry (MACSQuant 10, Miltenyi Biotec, Madrid, Spain). .. Macrophages were gated according to their forward- and side-scatter characteristics and data were analyzed by MACSQuantify software (Miltenyi Biotec, Bergisch Gladbach, Germany).

    Article Title: Chronic Alcohol Consumption Enhances the Differentiation Capacity of Hematopoietic Stem and Progenitor Cells into Osteoclast Precursors.
    Article Snippet: E-mail: ilhem.messaoudi@uky. edu.. Chronic alcohol consumption (CAC) is associated with an enhanced risk of bone fracture, reduced bone density, and osteoporosis.. Previous studies, using a rhesus macaque model of voluntary ethanol consumption, have shown that CAC induces functional, transcriptomic, and epigenomic changes in hematopoietic stem and progenitor cells (HSPCs) and their resultant monocytes/macrophages, skewing them toward a hyper-inflammatory response.

    other:

    Article Title: Unveiling spatial complexity in solid tumor immune microenvironments through multiplexed imaging
    Article Snippet: CD64 , REA987 , 50 , 130-116-195 , FITC , Miltenyi Biotec.



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